Revealing nourishing kidney drugs in the culture and differentiation of stem cells

doi:10.3969/j.issn.2095-4344.2013.14.021

Abstract

Abstract:

BACKGROUND: The continuous improvement of stem cell transplantation and understanding of the traditional Chinese medicine theory can strengthen the research regarding the effect of nourishing kidney drugs on the differentiation of stem cells.
OBJECTIVE: To investigate the interventional effect of nourishing kidney drugs on stem cell culture and transplantation.
METHODS: The relative studies on different categories of single herb or compound nourishing kidney drugs for the proliferation, differentiation and induction of stem cells during stem cell transplantation for the treatment of diseases were collected to analyze the experimental data, and to evaluate the interventional effect of nourishing kidney drugs on the stem cell culture and transplantation based on serum pharmacology.
RESULTS AND CONCLUSION: According to the basic research on nourishing kidney drugs and stem cells, and based on the existing new theory, the basic theoretical research was deepened continuously, in order to provide scientific theoretical guidance for the research on nourishing kidney drugs and stem cells. Based on the researches on the proliferation, differentiation and transdifferentiation mechanism of stem cells, the nourishing kidney drugs that could regulate stem cell growth were screened out. Then, combined with the achievement obtained from the clinical application, the single herb or compound nourishing kidney drugs were selected targetedly, and the interventional effect of the drugs on stem cell transplantation in the treatment of major diseases was analyzed.

Key words: stem cells, stem cell academic discussion, nourishing kidney drugs, bone marrow-derived stem cells, bone marrow stromal cells, bone marrow mesenchymal stem cells, hematopoietic stem cells, neural stem cells, hepatocyte, osteoblasts, animal experiments, proliferation and differentiation, immunohistochemistry, induction, in vitro culture, angiogenesis, provincial grants-supported paper

CLC Number:

R318

Gao Yue-cai, Li Meng, Li Rui-yu, Sun Yan-fu. Revealing nourishing kidney drugs in the culture and differentiation of stem cells [J]. Chinese Journal of Tissue Engineering Research, 2013, 17(14): 2609-2616.

Figures/Tables 4

2.2.2 补肾中药诱导干细胞向肝细胞分化的作用相关研究目前补肾中药或补肾中药成分诱导干细胞分化为肝细胞的报道很少，现有的报道表明，补肾中药促进干细胞分化为肝细胞的研究分为利用有补气作用的单味中药来体外诱导骨髓干细胞向肝细胞分化，应用血清药理学原理，利用具有补肾活血功效的复方中药血清，促进骨髓干细胞向肝细胞的分化[36]。李瀚旻等[37]为观察补肾中药左归丸含药血清对骨髓间质细胞分化为肝细胞的影响。对大鼠随机分为对照组、补肾中药组，制作药物血清，以体外肝细胞培养上清为诱导条件培养液，采用贴壁分离法纯化大鼠骨髓间质细胞，分别于诱导第0，7，14，21，28天免疫细胞化学法检测肝细胞标志物甲胎蛋白、细胞角蛋白18及白蛋白阳性细胞率等。结果发现：①诱导第0天，各组甲胎蛋白、白蛋白阳性细胞呈极少数表达，细胞角蛋白18及糖原均未见阳性细胞表达。②肝细胞生长因子加左归丸组、条件培养液加左归丸组在诱导第7，14，21，28天白蛋白、细胞角蛋白18、糖原的阳性细胞率均显著高于其他组，甲胎蛋白阳性细胞率于诱导第7天时增高显著，此外各时间点又明显降低。③与肝细胞生长因子加左归丸组比较，条件培养液加左归丸组白蛋白阳性细胞率在诱导第14，21，28天时显著升高，甲胎蛋白阳性细胞率在诱导第7天时显著升高，至诱导第14，21，28天则明显下降。因此，左归丸含药血清能够影响骨髓间质细胞向肝系细胞转化过程中的肝细胞标志物，从而提高骨髓间质细胞向肝细胞的转化率。万荣等[38]为观察剔毒护肝方及其拆方含药血清对大鼠骨髓干细胞增殖作用和向肝系分化的影响。对大鼠随机分为生理盐水对照组、剔毒护肝方组，制作药物血清，使用密度梯度离心和贴壁筛选相结合，分离骨髓干细胞，通过噻唑氮蓝比色法检测含药血清处理48 h时骨髓干细胞的增殖率，采用免疫组化法检测骨髓干细胞肝系标志甲胎蛋白、白蛋白的表达情况，诱导后甲胎蛋白和白蛋白放射免疫定量检测结果比较，见表1。"

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